We report positive results from our Phase I multiple ascending dose, open-label safety study of the Notch Inhibitor LY3056480 in 15 patients with mild to moderate sensorineural hearing loss (SNHL). LY3056480 mediated Notch inhibition is aimed at regeneration of inner ear sensory hair cells that are lost with advancing age. The study was conducted at University College London and University College London Hospitals and focused on the safety and tolerability of LY3056480 in patients with adult onset mild to moderate SNHL. Dose escalation within the Phase I clinical trial demonstrated that trans-tympanic injection (3 administrations, one week apart) at the highest dose of 250 micrograms of LY3056480 was safe and well tolerated. No safety issues were reported.